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Original Research Article | OPEN ACCESS

Study on the influence of sulfur fumigation on chemical constituents of Angelicae dahuricae Radix (Baizhi)

Yun-Bin Jiang1, Xiao-Lin Lu1,2, Wei Peng1, Wei Deng1, Yu-Ying Ma1

1College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137; 2Sichuan Agricultural University, Chengdu 611130, PR China.

For correspondence:-  Yu-Ying Ma   Email: cdtcmma@163.com   Tel:+8613678189939

Received: 19 December 2014        Accepted: 14 April 2015        Published: 26 May 2015

Citation: Jiang Y, Lu X, Peng W, Deng W, Ma Y. Study on the influence of sulfur fumigation on chemical constituents of Angelicae dahuricae Radix (Baizhi). Trop J Pharm Res 2015; 14(5):815-821 doi: 10.4314/tjpr.v14i5.11

© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To study the influence of different sulfur fumigation time and dosage on the chemical constituents of Baizhi (Angelicae dahurica Radix).
Methods: The relationship of chemical constituents in Baizhi with different sulfur fumigation time and dosages was evaluated by high performance liquid chromatography (HPLC) fingerprint and chemometrics methods, including similarity analysis (SA), hierarchical clustering analysis (HCA) and principal component analysis (PCA). HPLC was carried out on a Diamonil C18 column; linear gradient elution was performed with acetonitrile and water; column temperature, injection volume of sample, flow rate, detection wavelength and testing time were 30 °C, 20 μL, 1 mL/min, 310 nm and 75 min respectively.
Results: The chemical constituents of Baizhi significantly (p < 0.05) decreased after sulfur fumigation though sulfur fumigation time and dosage were at low levels – 2 h and 25 g (sulphur)/10 kg (Baizhi), respectively.
Conclusion: Sulfur fumigation is not a desirable method for field processing of Baizhi even when sulfur fumigation time and dosage were short and small.

Keywords: Baizhi, Angelicae dahurica, Sulfur fumigation, HPLC-DAD, Fingerprint, Chemometrics methods

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Thompson Reuters (ISI): 0.523 (2021)
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